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tween 20 pbs t  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tween 20 pbs t
    Tween 20 Pbs T, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/saline+tween+20/Phosphate+Buffered+Saline+with+Tween+20/pmc12999298-65-8-30
    Average 96 stars, based on 823 article reviews
    tween 20 pbs t - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Saline:

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection
    Article Snippet: The membranes were pretreated in Tris-buffered saline-Tween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. .. The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection.
    Article Snippet: The membranes were pretreated in Tris-buffered salineTween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. miRNA microarray analysis The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Kidney injury in a murine hemorrhagic shock/resuscitation model is alleviated by sulforaphane’s anti-inflammatory and antioxidant action
    Article Snippet: .. The PVDF membrane was immersed in the solution of 5% BSA-tris-buffered saline-Tween 20 (TBST; CST, Danvers, MA, USA) to block nonspeci c antigens for 1 h. The primary antibody dilution was used for incubation overnight at 4°C. .. Primary antibodies used were TIM-1 (1:1000, MBS2032350; Mybiosource) and Nrf2 (1:500, ab137550; Abcam).

    Article Title: HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression.
    Article Snippet: The protein concentration was quantified by a BCA Protein Assay Kit (P0012, Beyotime, China) and equal amounts of proteins were analyzed on One-Step PAGE Gel (8%–12 %, Vazyme, China) and then transferred onto PVDF membranes (Millipore, IPVH00010). .. The proteins were then blocked with 5 % bovine serum albumin (BSA, 4240GR500, BioFroxx, L. Zhang et al. Redox Biology 68 (2023) 102939 Germany) in Tris-buffered saline-Tween 20 (TBST, 9997, CST, USA) and then incubated with primary antibodies. .. The anti-bodies used were as following: GPX4 (A11243, 1:2000,ABclonal, China); HDAC1 (1:1000, #5356P, Cell Signaling Technology, USA); HDAC2 (1:1000, #5113P, Cell Signaling Technology, USA); HDAC3 (1:2000, A16462, ABclonal, China); HDAC8 (1:1000, A5829, ABclonal, China); 4-Hydroxynonenal (ab46545, 1:500, Abcam, UK); KLF5 (AF04622, 1:1000, AiFang biological, China); E-cadherin (GB11082, 1:2000) and type 1 collagen (GB11022-2, 1:500) from Servicebio, China; α-SMA (sc-32251, 1:500, Santa Cruz, USA); histone 3 (4499T, 1:1000, Cell Signaling Technology, USA); acetylated histone 3 (Ac-H3, 06–599, 1:1000, Milli-pore, USA); GAPDH (60004-1-Ig, 1:5000, Proteintech, USA).

    Article Title: CD2AP is a potential prognostic biomarker of renal clear cell carcinoma
    Article Snippet: .. After closure with 5% BSA in Tris‐buffered saline‐Tween‐20, the membranes were incubated with CD2AP (Cell Signaling Technology, 5478S) and GAPDH (abcam, ab9485) primary antibodies at 4°C overnight, dilution‐labeled HRP secondary antibody was added and incubated for 1 h at room temperature and DAB developed. ..

    Article Title: A CB2 Agonist Alleviates Liver Fibrosis by Downregulating the ERK1/2 Signalling Pathway in Mouse Hepatic Fibrosis Model
    Article Snippet: .. Membranes were blocked in 5% nonfat milk in Tris-buffered saline/Tween 20 for 2 hours at room temperature and then incubated with antibodies speci c for pERK1/2 (1:2000, CST, USA), ERK1/2 (1:500, Wanlei, China), p-CREB (1:400, Cell Signaling Technology (CST), USA), CREB (1:250, Wanlei, China), Bcl-2 (1:250, Wanlei, China), Bax (1:250, Wanlei, China), cleaved caspase-3 (1:500, CST, USA), and CB2 (1:400, Abcam, USA). ..

    Article Title: HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression
    Article Snippet: The protein concentration was quantified by a BCA Protein Assay Kit (P0012, Beyotime, China) and equal amounts of proteins were analyzed on One-Step PAGE Gel (8%–12 %, Vazyme, China) and then transferred onto PVDF membranes (Millipore, IPVH00010). .. The proteins were then blocked with 5 % bovine serum albumin (BSA, 4240GR500, BioFroxx, Germany) in Tris-buffered saline-Tween 20 (TBST, 9997, CST, USA) and then incubated with primary antibodies. .. The anti-bodies used were as following: GPX4 (A11243, 1:2000,ABclonal, China); HDAC1 (1:1000, #5356P, Cell Signaling Technology, USA); HDAC2 (1:1000, #5113P, Cell Signaling Technology, USA); HDAC3 (1:2000, A16462, ABclonal, China); HDAC8 (1:1000, A5829, ABclonal, China); 4-Hydroxynonenal (ab46545, 1:500, Abcam, UK); KLF5 (AF04622, 1:1000, AiFang biological, China); E-cadherin (GB11082, 1:2000) and type 1 collagen (GB11022-2, 1:500) from Servicebio, China; α-SMA (sc-32251, 1:500, Santa Cruz, USA); histone 3 (4499T, 1:1000, Cell Signaling Technology, USA); acetylated histone 3 (Ac-H3, 06–599, 1:1000, Milli-pore, USA); GAPDH (60004-1-Ig, 1:5000, Proteintech, USA).

    Incubation:

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection
    Article Snippet: The membranes were pretreated in Tris-buffered saline-Tween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. .. The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection.
    Article Snippet: The membranes were pretreated in Tris-buffered salineTween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. miRNA microarray analysis The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Kidney injury in a murine hemorrhagic shock/resuscitation model is alleviated by sulforaphane’s anti-inflammatory and antioxidant action
    Article Snippet: .. The PVDF membrane was immersed in the solution of 5% BSA-tris-buffered saline-Tween 20 (TBST; CST, Danvers, MA, USA) to block nonspeci c antigens for 1 h. The primary antibody dilution was used for incubation overnight at 4°C. .. Primary antibodies used were TIM-1 (1:1000, MBS2032350; Mybiosource) and Nrf2 (1:500, ab137550; Abcam).

    Article Title: HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression.
    Article Snippet: The protein concentration was quantified by a BCA Protein Assay Kit (P0012, Beyotime, China) and equal amounts of proteins were analyzed on One-Step PAGE Gel (8%–12 %, Vazyme, China) and then transferred onto PVDF membranes (Millipore, IPVH00010). .. The proteins were then blocked with 5 % bovine serum albumin (BSA, 4240GR500, BioFroxx, L. Zhang et al. Redox Biology 68 (2023) 102939 Germany) in Tris-buffered saline-Tween 20 (TBST, 9997, CST, USA) and then incubated with primary antibodies. .. The anti-bodies used were as following: GPX4 (A11243, 1:2000,ABclonal, China); HDAC1 (1:1000, #5356P, Cell Signaling Technology, USA); HDAC2 (1:1000, #5113P, Cell Signaling Technology, USA); HDAC3 (1:2000, A16462, ABclonal, China); HDAC8 (1:1000, A5829, ABclonal, China); 4-Hydroxynonenal (ab46545, 1:500, Abcam, UK); KLF5 (AF04622, 1:1000, AiFang biological, China); E-cadherin (GB11082, 1:2000) and type 1 collagen (GB11022-2, 1:500) from Servicebio, China; α-SMA (sc-32251, 1:500, Santa Cruz, USA); histone 3 (4499T, 1:1000, Cell Signaling Technology, USA); acetylated histone 3 (Ac-H3, 06–599, 1:1000, Milli-pore, USA); GAPDH (60004-1-Ig, 1:5000, Proteintech, USA).

    Article Title: CD2AP is a potential prognostic biomarker of renal clear cell carcinoma
    Article Snippet: .. After closure with 5% BSA in Tris‐buffered saline‐Tween‐20, the membranes were incubated with CD2AP (Cell Signaling Technology, 5478S) and GAPDH (abcam, ab9485) primary antibodies at 4°C overnight, dilution‐labeled HRP secondary antibody was added and incubated for 1 h at room temperature and DAB developed. ..

    Article Title: A CB2 Agonist Alleviates Liver Fibrosis by Downregulating the ERK1/2 Signalling Pathway in Mouse Hepatic Fibrosis Model
    Article Snippet: .. Membranes were blocked in 5% nonfat milk in Tris-buffered saline/Tween 20 for 2 hours at room temperature and then incubated with antibodies speci c for pERK1/2 (1:2000, CST, USA), ERK1/2 (1:500, Wanlei, China), p-CREB (1:400, Cell Signaling Technology (CST), USA), CREB (1:250, Wanlei, China), Bcl-2 (1:250, Wanlei, China), Bax (1:250, Wanlei, China), cleaved caspase-3 (1:500, CST, USA), and CB2 (1:400, Abcam, USA). ..

    Article Title: HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression
    Article Snippet: The protein concentration was quantified by a BCA Protein Assay Kit (P0012, Beyotime, China) and equal amounts of proteins were analyzed on One-Step PAGE Gel (8%–12 %, Vazyme, China) and then transferred onto PVDF membranes (Millipore, IPVH00010). .. The proteins were then blocked with 5 % bovine serum albumin (BSA, 4240GR500, BioFroxx, Germany) in Tris-buffered saline-Tween 20 (TBST, 9997, CST, USA) and then incubated with primary antibodies. .. The anti-bodies used were as following: GPX4 (A11243, 1:2000,ABclonal, China); HDAC1 (1:1000, #5356P, Cell Signaling Technology, USA); HDAC2 (1:1000, #5113P, Cell Signaling Technology, USA); HDAC3 (1:2000, A16462, ABclonal, China); HDAC8 (1:1000, A5829, ABclonal, China); 4-Hydroxynonenal (ab46545, 1:500, Abcam, UK); KLF5 (AF04622, 1:1000, AiFang biological, China); E-cadherin (GB11082, 1:2000) and type 1 collagen (GB11022-2, 1:500) from Servicebio, China; α-SMA (sc-32251, 1:500, Santa Cruz, USA); histone 3 (4499T, 1:1000, Cell Signaling Technology, USA); acetylated histone 3 (Ac-H3, 06–599, 1:1000, Milli-pore, USA); GAPDH (60004-1-Ig, 1:5000, Proteintech, USA).

    Microarray:

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection.
    Article Snippet: The membranes were pretreated in Tris-buffered salineTween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. miRNA microarray analysis The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA). ..

    Expressing:

    Article Title: MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection.
    Article Snippet: The membranes were pretreated in Tris-buffered salineTween 20 with 5% dry milk for 1 h and then incubated overnight at 4 °C with primary antibodies (anti-CD9 clone 12A12, Cosmo Bio, Tokyo, Japan; anti-L1CAM, clone UJ127, Abcam, Cambridge, UK). .. The membranes were washed three times with Tris-buffered saline-Tween 20 and incubated with the corresponding secondary antibodies (horseradish peroxidase-linked anti-mouse IgG, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature. miRNA microarray analysis The expression levels of 2588 miRNAs were studied using a SurePrint G3 Human miRNA microarray 8 × 60K (Agilent Technologies, Santa Clara, CA, USA). ..

    Membrane:

    Article Title: Kidney injury in a murine hemorrhagic shock/resuscitation model is alleviated by sulforaphane’s anti-inflammatory and antioxidant action
    Article Snippet: .. The PVDF membrane was immersed in the solution of 5% BSA-tris-buffered saline-Tween 20 (TBST; CST, Danvers, MA, USA) to block nonspeci c antigens for 1 h. The primary antibody dilution was used for incubation overnight at 4°C. .. Primary antibodies used were TIM-1 (1:1000, MBS2032350; Mybiosource) and Nrf2 (1:500, ab137550; Abcam).

    Article Title: Autophagy inhibitors 3-MA and BAF may attenuate hippocampal neuronal necroptosis after global cerebral ischemia-reperfusion injury in male rats by inhibiting the interaction of the RIP3/AIF/CypA complex.
    Article Snippet: 1Department of Neurology, First Affiliated Hospital of Anhui Medical University, Hefei, China 2Key Laboratory of Noncoding RNA Transformation Research of Anhui Higher Education Institutes, First Affiliated Hospital of Wannan Medical College, Wuhu, China 3Department of Neurology, First Affiliated Hospital of Wannan Medical College, Wuhu, China 4Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China 5School of Basic Medical Sciences, Anhui Medical University, Hefei, China 6Dr.. Neher's Biophysics Laboratory for Innovative Drug Discovery, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China

    Blocking Assay:

    Article Title: Kidney injury in a murine hemorrhagic shock/resuscitation model is alleviated by sulforaphane’s anti-inflammatory and antioxidant action
    Article Snippet: .. The PVDF membrane was immersed in the solution of 5% BSA-tris-buffered saline-Tween 20 (TBST; CST, Danvers, MA, USA) to block nonspeci c antigens for 1 h. The primary antibody dilution was used for incubation overnight at 4°C. .. Primary antibodies used were TIM-1 (1:1000, MBS2032350; Mybiosource) and Nrf2 (1:500, ab137550; Abcam).



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